About the ELMI

ELMI was created in 2001 to establish a unique communication network between European scientists working in the field of light microscopy and the manufacturers of their equipment.

Improve your imaging skills – from sample preparation to image analysis, 07-22nd April, Stockholm, Sweden

stockholmMicroscopy: improve your imaging skills –

from sample preparation to image analysis

07-22nd April 2016

Karolinska Institute, Stockholm, Sweden

Target audience:

The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples and want to improve their skills.

Two courses run in parallel: the full course contains lectures and workshops while the short course contains only lectures.

 Aim of the course:

The focus is on providing the students with enough theoretical and practical knowledge so that, when they go back to their lab, they are able to properly use the available hardware and so that they fully understand each parameter they need to set in the software. The aim is to provide them with the tools to acquire on any microscope, images that exactly match their samples and answer their scientific questions in a reliable way.

Participants will learn the theory and many practical tricks about the parameters and hardware used in confocal imaging, how to identify and avoid imaging artifacts, deal with the challenges of imaging fluorescent volumes, get started with automated image analysis, as well as how to handle scientific images for publication.

They will also hear many tricks about fixation, mounting and handling of their sample in a way that is optimal for imaging and they will learn about more advanced techniques like two-photon microscopy, super resolution and spectral imaging.

They will get personalized expert feedback as well as a full analysis of their sample preparation and imaging strategy, using their own sample.

Learning outcomes for the full course:

At the end of the course, students will be able to:


Registration fee:

Registration is free


Number of participants:

Theory and workshops: max. 16. There are only 2 places left!

Theory only: unlimited


Theory and workshops: 9-17 every day (shorter the last day)

Theory only: 10-15 every day (shorter the last day, free on 21st)

 Contact and venue:

Sylvie Le Guyader

Live Cell Imaging facility, Nikon Center of Excellence

Karolinska Institutet

Hälsövägen 7

14157 Huddinge, Sweden


Facility website