EMBL course Super-resolution microscopy: time-resolved STED nanoscopy , 8-15 July 2022, virtual
This six-day comprehensive microscopy course focuses on stimulated emission depletion microscopy (STED) and its application to biological questions. Through lectures, tutorials, demonstrations and practical workshops, the course provides in-depth knowledge on all aspects of STED Nanoscopy. It covers the fundamentals of STED microscopy, different technical implementations and has a strong emphasis on sample preparation – including choice of fluorophores and refractive index matching, as well as image acquisition strategies for fixed samples and living cells. Students will have the opportunity for exercises on state-of the art STED microscopes in which they will acquire high quality nanoscopy data. The faculty consists of Invited guest researchers, experts from the EMBL and Leica Microsystems.
This course is designed for researchers at the early stages of their career in the life sciences (e.g. pre or postdoctoral level), ideally with access to a STED microscope. The participants should have an immediate need to apply the trained skills for their biological questions. The course is strongly centered on practical aspects of STED microscopy and is therefore especially suited for people who rely on STED nanoscopy for their research.
The course will cover the entire range of skills needed to apply STED nanoscopy in biology. The following topics will be covered in lectures, tutorials as well as in practical workshops:
- Sample preparation for STED microscopy, including choice of fluorophores and refractive index matching
- Technical implementations – CW-STED, pulsed-STED, gated-STED, time-resolved STED (tauSTED)
- Multi-Color 2D and 3D STED microscopy
- Image acquisition strategies for live-cell STED
For additional information please check the course page.
Application deadline 2 May 2022