About the ELMI

ELMI was created in 2001 to establish a unique communication network between European scientists working in the field of light microscopy and the manufacturers of their equipment.

Advanced fluorescence imaging techniques, 7-12 May 2023, EMBL Heidelberg, Germany

Course overview

This practical course will cover advanced light microscopy techniques and participants will learn how to derive qualitative and quantitative insights on molecular mechanisms in cells and developing organisms. Invited guest researchers together with microscopy experts from the EMBL will foster an intense information flow with a balance of lectures and practical workshops. The focus of the course will be on the use of fluorescence microscopy to obtain information about localisation and functions of proteins and other bio-molecules at different spatial scales.


This course is directed towards researchers in the life sciences with access to light microscopy equipment, who have an immediate need to apply the techniques they will learn to solve biological problems at the cellular and molecular level in vivo. As the course will be primarily focused on the advanced techniques, we expect participants to have basic knowledge of light microscopy.


The topics will include fluorescence microscopy techniques such as fast time-lapse microscopy of living cells, spinning disk and point-scanning confocal microscopy, light sheet microscopy of cleared samples, photo-manipulation as a tool, multicolour single-molecule localization microscopy (SMLM) with spectral demixing, expansion microscopy, FRET-based techniques. For demanding applications, we will cover the aspects of image processing and analysis and the possibilities of microscope automation. The modules will cover all necessary knowledge and demonstrate tools to conduct similar pipelines at your home lab enabling you to answer emerging biological questions.

Learning outcomes

This course is kindly supported by the EMBL Advanced Light Microscopy Facility.
This course is organised in cooperation with Evident.

For additional information please check the course pages.
Registration deadline: 12 Feb 2023