About the ELMI

ELMI was created in 2001 to establish a unique communication network between European scientists working in the field of light microscopy and the manufacturers of their equipment.

Confocal Microscopy Course 12-15 September 2023, University of York, UK

Running for over 19 years, with over 400 delegates to date and with 100% recommended feedback, the course is run in the Bioscience Technology Facility, University of York which is based in the welcoming, friendly and historic city of York. York itself is  one of the UK’s best tourist places due to its Roman, Viking, Medieval and current importance with buildings, streets and museums to match (see https://https://www.visityork.org/).

The course includes many varied sample types that are used to best illustrate various practical aspects of confocal microscopy whilst also demonstrating a range of applications.

Tutorials and practical sessions will cover both basic and advanced confocal microscopy.

The course will enable participants to realise the potential behind many developing confocal techniques and realise the simplicity of applying the techniques to a broad range of sample types and fluorescent labels. Both novice and more experienced users welcome. This course is 100% recommended from previous course delegates and comments can be seen below.

Preliminary Programme

DAY AIMS

Day 1: Basics, Familiarisation, x,y,z and multicolour imaging.

Day 2: Live-cell imaging with spinning disk confocal microscopy, FRAP on laser scanning confocal.

Day 3: Advanced Techniques covering spectral unmixing and FRET with fluorescent proteins.

Day 4: Super-resolution and other advances in confocal and associated microscopy approaches. More samples to explore airy scanning approaches and confocal limits, and time for participants own samples.

Thursday evening will include a highlights talk on new developments and course meal.

The course will utilise many different sample types ranging from cultured monolayers to plant cells. A diverse range of fluorescent labels will also be used, which will include various fluorescent proteins and classic antibody labels. Over the four days, at least four confocal microscopes (Zeiss LSM 710/880/2 x 980 Airyscan FAST on both invert and upright microscopes, and the Andor Dragonfly) will be utilised.

Accommodation (en-suite), breakfast and lunches will be provided for the length of the course.  Evening meals will be provided on both Tuesday and Thursday evenings.

Places are limited to 12 participants to permit full hands-on practice, so please book early to avoid disappointment.

For further information, please visit the course website at: https://www.york.ac.uk/biology/technology-facility/tfcourses/confocal-course/