About the ELMI

ELMI was created in 2001 to establish a unique communication network between European scientists working in the field of light microscopy and the manufacturers of their equipment.

Confocal Microscopy Course, 19-22 March 2024, University of York, UK

From the fundamentals to advanced confocal microscopy our highly successful international 4-Day Intensive Hands-On Course will run again on 19-22 March 2024. Have fun whilst you learn! A very interactive course.

Website: https://www.york.ac.uk/biology/technology-facility/tfcourses/

Now in its 21st year, with over 450 delegates to date and with100% recommended feedback, the course is run in the BioscienceTechnology Facility, University of York which is based in the welcoming, friendly and historic city of York. York itself is one of the UK’s best tourist places due to its Roman, Viking, Medieval and current importance with buildings, streets and museums to match (see https://https://www.visityork.org/).

The course includes many varied sample types that are used to best illustrate various practical aspects of confocal microscopy whilst also demonstrating a range of applications.

Tutorials and practical sessions will cover both basic and advanced confocal microscopy. The course will enable participants to realise the potential behind many developing confocal techniques and realise the simplicity of applying the techniques to a broad range of sample types and fluorescent labels. Both novice and more experienced users are welcome. This course is 100% recommended from previous course delegates and comments can be seen below.

Preliminary Programme

DAY AIMS

Day 1: Basics, Familiarisation, x,y,z and multicolour imaging.

Day 2: Live-cell imaging with spinning disk confocal microscopy, FRAP on laser scanning confocal.

Day 3: Advanced Techniques covering spectral unmixing and FRET with fluorescent proteins.

Day 4: Super-resolution and other advances in confocal and associated microscopy approaches. More samples to explore airy scanning approaches and confocal limits, and time for participants’ own samples.

Thursday evening will include a highlights talk on new developments and a course meal.

The course will utilise many different sample types ranging from cultured monolayers to plant cells. A diverse range of fluorescent labels will also be used, which will include various fluorescent proteins and classic antibody labels. Over the four days, the course usually uses Zeiss LSM 980’s and 880 on both invert and upright microscopes, and the Andor Spinning Disk with potential for more options. However the course remains as platform agnostic as possible and is useful for all confocal microscope users.

Accommodation (en-suite), breakfast and lunches will be provided for the length of the course.  Evening meals will be provided on both Tuesday and Thursday evenings.

Places are limited to 12 participants to permit full hands-on practice, so please book early to avoid disappointment. For further information, please visit the course website at:

https://www.york.ac.uk/biology/technology-facility/tfcourses/ or contact +44 (0)1904 328821, btf-enquiries@york.ac.uk