About the ELMI

ELMI was created in 2001 to establish a unique communication network between European scientists working in the field of light microscopy and the manufacturers of their equipment.

Microscopy: improve your imaging skills – from sample preparation to image analysis, 17 Jan – 09 Feb 2017, Karolinska Institute, Stockholm, Sweden

course webpage


Target audience:

The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples and want to improve their skills.

Two courses run in parallel: the full course contains lectures and workshops while the short course contains only lectures.

Aim of the course:

The focus is on providing the students with enough theoretical and practical knowledge so that, when they go back to their lab, they are able to properly use the available hardware and so that they fully understand each parameter they need to set in the software. The aim is to provide them with the tools to acquire on any microscope, images that exactly match their samples and answer their scientific questions in a reliable way.

Participants will learn the theory and many practical tricks about the parameters and hardware used in confocal imaging, how to identify and avoid imaging artifacts, deal with the challenges of imaging fluorescent volumes, get started with automated image analysis, as well as how to handle scientific images for publication.

They will also hear many tricks about fixation, mounting and handling of their sample in a way that is optimal for imaging and they will learn about more advanced techniques like two-photon microscopy, super resolution and spectral imaging.

They will get personalized expert feedback as well as a full analysis of their sample preparation and imaging strategy, using their own sample.

Learning outcomes for the full course:

At the end of the course, students will be able to:

 Registration fee:

Registration is free

Number of participants:

Theory and workshops: max. 16
Theory only: unlimited


Lectures and workshops: 9-17 every day
Lectures only: 10-15 every day

Contact and venue:

Sylvie Le Guyader
Live Cell Imaging facility, Nikon Center of Excellence
Karolinska Institutet
Hälsövägen 7
14157 Huddinge, Sweden
Facility website

course webpage