3D microscopy imaging with clearness-1st edition, 6-8 November 2023, i3S Porto, Portugal
Light sheet fluorescence microscopy (LSFM) is the preferred technology for in-depth imaging of large optically cleared samples and 3D observation of live specimens with high spatiotemporal resolution. LSFM has the extraordinary optical sectioning capability, axial resolution, restrictive phototoxicity, photobleaching, and high acquisition speed, and when combined with optical clearing methods, can overcome the difficulties of imaging specimens that are not typically transparent. Clearing techniques have been used since the 20th century but, because multiple tissue-clearing protocols have been developed, choosing the most appropriate approach can be challenging. Something to keep in mind is that each sample and application will always require hands-on optimization of the clearing method of choice.
This workshop offers an in-depth presentation of the different clearing methods available, combined with hands-on sessions where participants will be able to perform tissue clearing and sample imaging using LSFM, and confocal microscopy for comparing technologies.
Target audience: The course is designed for Ph.D. students, early career researchers plus all researchers interested in clearing techniques and in acquiring skills in light sheet microscopy.
Course duration: 24 hours
Teaching Language: English
Certificate: A certificate of attendance will be assigned to participants who attend a minimum of 80% of the course duration
Course Coordination: Maria Azevedo, i3S; Paula Sampaio, i3S; Jacques Paysan, Zeiss
More information and registration at https://www.i3s.up.pt/training-detail.php?v=281