Bioimaging Experts, Center of Cellular Nanoanalytics, University of Osnabrueck, Germany


The Center of Cellular Nanoanalytics (CellNanOs) at the University of Osnabrück seeks two

Bioimaging Experts

for the operation and scientific support of the DFG-funded bioimaging core facility

iBiOs (integrated Bioimaging Facility Osnabrück)

The positions are to be filled at earliest convenience. Payment is according Germany pay scale E13 TV-L, full time. Contracts are initially fixed for 3 years, and extensions are anticipated.

Overview:

The Center for Cellular Nanoanalytics Osnabrück (CellNanOs, www.cellnanos.uni-osnabrueck.de) is an interdisciplinary research center of the schools of Biology/Chemistry, Physics, Human Sciences and Mathematics/Informatics at the University of Osnabrück promoting research at the interface between nanosciences and cell biology. CellNanOs aims to provide a dedicated bioanalytical infrastructure for unraveling basic functional principles of cellular microcompartments in the context of a variety of physiological processes and biological organisms. This overarching topic of the Biology Department in Osnabrück has been the focus of the collaborative research center SFB 944 since 2011 (www.biologie.uni-osnabrueck.de/sonderforschungsbereich/sfb_944_home.html).

Advanced high and super-resolution fluorescence and electron microscopy are key methodologies within the CellNanOs and SFB 944, which are funded by the DFG through the core facility initiative iBiOs since early 2017. The CellNanOs was rigorously planned to create ideal conditions for the application and integration of advanced light and electron microscopy techniques. Next to single molecule localization microscopy up to four colors, a clone of the lattice light sheet microscope (LLSM) from the Betzig lab was recently successfully installed. Laboratories with ultrahigh vibrational stability and climate control will be closely interconnected to versatile sample preparation facilities and cell culture.

Tasks:

The main scope of the iBiOs is to provide dedicated techniques for visualizing the nanoscopic spatiotemporal organization of cellular microcompartments in cells, tissues and small organisms.

The scientist on position iBIOS1/2017 will adapt lattice light-sheet microscopy to various cell types (plant, insect and mammalian cell lines), tissues and small organisms to allow for volumetric imaging at highest spatiotemporal resolution. For this purpose, he/she will establish 3D super-resolution techniques based on structured illumination microscopy (SIM and non-linear SIM) and single molecule localization microscopy (PALM/STORM, PAINT) for LLSM. In close collaboration with the electron microscopy unit (serial block face SEM, FIB-SEM, EM-tomography, etc.), he/she will implement correlative light and microscopy (CLEM) approaches.

The scientist on position iBIOS2/2017 is mainly responsible for developing tailored image and data processing algorithms for the visualization, segmentation and quantitative spatiotemporal analysis of complex multidimensional data sets from light and electron microscopy. He/she needs to establish effective workflows with user-friendly software and high-performance matching hardware for big data sets (10-100 GB).

Both scientists are expected to closely collaborate with the existing iBiOs-team (www.calm.uni-osnabrueck.de) in order to provide support in planning and implementation of light and electron microscopy experiments as well as dedicated data analyses. Established workflows for sample preparation, data acquisition and application of software tools should be disseminated by regular user workshops.

Application deadline: 13 July 2017

For additional information please check the job offer.